Dangerous Bacterium Hosts Genetic Remnant of Life’s Distant Past
New Haven, Conn. — Within a dangerous stomach bacterium, Yale University researchers have discovered an ancient but functioning genetic remnant from a time before DNA existed, they report in the August 13 issue of the journal Science.
To the surprise of researchers, this RNA complex seems to play a critical role in the ability of the organism to infect human cells, a job carried out almost exclusively by proteins produced from DNA’s instruction manual.
“What these cells are doing is using ancient RNA technology to control modern gene expression,” said Ron Breaker, the Henry Ford II Professor of Molecular, Cellular and Developmental Biology at Yale, investigator for the Howard Hughes Medical Institute and senior author of the study.
In old textbooks, RNA was viewed simply as the chemical intermediary between DNA’s instruction manual and the creation of proteins. However, Breaker’s lab has identified the existence and function of riboswitches, or RNA structures that have the ability to detect molecules and control gene expression – an ability once believed to be possessed solely by proteins. Breaker and many other scientists now believe the first forms of life depended upon such RNA machines, which would have had to find ways to interact and carry out many of the functions proteins do today.
The new paper describes the complex interactions of two small RNA molecules and two larger RNA molecules that together influence the function of a self-splicing ribozyme, a structure many biologists had believed had no role other than to reproduce itself. The new study, however, suggests that in the pathogenic stomach bacterium Clostridium difficile, this RNA structure acts as a sort of sensor to help regulate the expression of genes, probably to help the bacterium manipulate human cells.
“They were though to be molecular parasites, but it is clear they are being harnessed by cells to do some good for the organism,” Breaker said.
This is the sort of RNA structure that would have been needed for life exist before the evolution of double-stranded DNA, with its instruction book for proteins that carry out almost all of life’s functions today. If proteins are necessary to carry out life’s functions, scientists need to explain how life arises without DNA’s recipe. The answer to the chicken or egg question is RNA machines such as the one identified in the new study, Breaker said.
“A lot of sophisticated RNA gadgetry has gone extinct but this study shows that RNA has more of the power needed to carry out complex biochemistry,” Breaker said. “It makes the spontaneous emergence of life on earth much more palatable.”
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